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United States Department of Agriculture

Agricultural Research Service

Title: Transposable Element-Mediated Transgenic Insects in the Screwworm Program

Authors
item ALLEN, MARGARET
item HANDLER, ALFRED
item BERKEBILE, DENNIS
item SKODA, STEVEN
item Scholl, Philip

Submitted to: Livestock Insect Worker's Conference Annual Meeting
Publication Type: Other
Publication Acceptance Date: May 4, 2002
Publication Date: June 23, 2002

Interpretive Summary: The following interpretive summary refers to an oral presentation given at the 2002 Annual Meeting of the Livestock Insect Worker's Conference. Transposable element (TE)-mediated transformation of non-drosophilid insects is becoming a viable and robust gene introduction method for many insects of economic and medical importance. Transformation may be used for gene identification and characterization, and for creating strains with genes encoding lethality or sterility. This technology now has been adapted for and applied to the primary screwworm, Cochliomyia hominivorax (Coquerel). Using a piggyBac TE system, a green fluorescent marker gene has been introduced to the germ line of a laboratory strain (P95) of screwworms, resulting in several transgenic lines of uniquely marked strains. The genetics and characterization of these new transgenic lines will be presented.

Technical Abstract: The following technical abstract refers to an oral presentation given at the 2002 Annual Meeting of the Livestock Insect Worker's Conference. Transposable element (TE)-mediated transformation of non-drosophilid insects is becoming a viable and robust gene introduction method for many insects of economic and medical importance. Transformation may be used for gene identification and characterization, and for creating strains with genes encoding lethality or sterility. This technology now has been adapted for and applied to the primary screwworm, Cochliomyia hominivorax (Coquerel). Using a piggyBac TE system, a green fluorescent marker gene has been introduced to the germ line of a laboratory strain (P95) of screwworms, resulting in several transgenic lines of uniquely marked strains. The genetics and characterization of these new transgenic lines will be presented.

Last Modified: 9/10/2014
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