Page Banner

United States Department of Agriculture

Agricultural Research Service

Title: Pollen Tube Localization Implies a Role in Pollen-Pistil Interactions for the Tomato Receptor-Like Protein Kinases Leprk1 and Leprk2

Authors
item Muschietti, Jorge - USDA/UCB PGEC
item Eyal, Yoram - USDA/UCB PGEC
item McCormick, Sheila

Submitted to: The Plant Cell
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 1, 1998
Publication Date: March 1, 1998

Interpretive Summary: We screened for pollen-specific kinase genes and isolated two structurally related receptor-like kinases RLKs from tomato, LePRK1 and LePRK2. The results suggest that LePRK1 and LePRK2 play different roles in postpollination events and that at least LePRK2 may mediate some pistil response.

Technical Abstract: We screened for pollen-specific kinase genes, which are potential signal transduction components of pollen-pistil interactions, and isolated two structurally related receptor-like kinases RLKs from tomato, LePRK1 and LePRK2. These kinases are similar to a pollen-expressed RLK from petunia, but they are expressed later during pollen development than is the petunia RLK. The abundance of LePRK2 increases when pollen germinates, but LePRK1 remains constant. Both LePRK1 and LePRK2 are localized to the plasma membrane/cell wall of growing pollen tubes. Both kinase domains have kinase activity when expressed in Escherichia coli. In phosphorylation assays with pollen membrane preparations, LePRK2, but not LePRK1, is phosphorylated, and the addition of tomato style, but not leaf, extracts to these membrane preparations results at least partially in specific dephosphorylation of LePRK2. Taken together, these results suggest that LePRK1 and LePRK2 play different roles in postpollination events and that at least LePRK2 may mediate some pistil response.

Last Modified: 10/20/2014
Footer Content Back to Top of Page