Page Banner

United States Department of Agriculture

Agricultural Research Service

Title: An Integrated Map for Maize

Authors
item Gardiner, J - UNIV OF MISSOURI
item Cone, K - UNIV OF MISSOURI
item McMullen, Michael
item Schaeffer, Mary
item Davis, G - UNIV OF MISSOURI
item Yim, Y - UNIV OF MISSOURI
item Soderlund, C - CLEMSON UNIVERSITY
item Wing, R - CLEMSON UNIVERSITY
item Chen, M - CLEMSON UNIVERSITY
item Coe Jr, Edward

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: January 10, 2002
Publication Date: January 10, 2002
Citation: GARDINER, J., CONE, K., MCMULLEN, M.D., POLACCO, M.L., DAVIS, G., YIM, Y., SODERLUND, C., WING, R., CHEN, M., COE JR, E.H. AN INTEGRATED MAP FOR MAIZE. PLANT AND ANIMAL GENOME. 2002. ABSTRACT. P. 186.

Technical Abstract: The Maize Mapping Project (MMP) is focused on developing genetic, physical, and database resources for the maize genome. A key resource being developed by the MMP is a well-integrated genetic and physical map that will expedite the identification of DNA sequences underlying key traits that have been genetically mapped. Towards this end, a high-resolution genetic map has been developed that contains over 1800 RFLP and SSR markers. A physical map consisting of 450,000 HindIII fingerprinted BACs from EcoRI (162 kb), MboI (165 kb), and Hind III (137 kb) libraries is well underway with completion expected in early 2002. To complete the task of integrating the two maps, several complementary approaches are being taken. AFLPs are being used on both the high-resolution genetic map and on BAC pools to link genetically mapped AFLPs to BACs on the physical map. Approximately 10,000 EST unigene clusters are being anchored to 165,000 BACs using 40 bp overgo sequences designed to hybridize to single- or low-copy regions. A portion of these EST unigene clusters contain previously mapped SSRs that will allow BAC contigs from the physical map to be linked to the genetic map. A portion of the BAC contigs with EST unigene anchors that contain no genetic mapping information will be localized to the genetic map by the development of single nucleotide polymorphism markers and their subsequent mapping on the high-resolution genetic map.

Last Modified: 9/23/2014
Footer Content Back to Top of Page