Submitted to: American Society of Animal Science Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 2002
Publication Date: July 21, 2002
Citation: Zuelke, K.A., Blomberg, L., Long, E.L., Sonstegard, T.S. 2002. Serial analysis of gene expression (sage) in preimplantation stage swine embryos. American Society of Animal Science Proceedings.
Serial Analysis of Gene Expression (SAGE) enables both qualitative and quantitative analysis of gene expression on a whole-transcriptome level (4). SAGE consists of extracting mRNA template from cells of interest and performing a series of manipulations to isolate and sequence a population of 10-12 base-pair nucleotide 'tags' (i.e. a SAGE library) wherein each tag is uniquely representative of an individual transcript represented in the original mRNA population. Bioinformatic analysis of SAGE tag sequence data identifies and quantitates the occurrence of each tag; the tag frequency is correlated to the frequency at which its respective transcript occurs in the mRNA population. We have constructed SAGE libraries representing in vivo-derived Day 11 and Day 12 stage swine embryos as part of an ongoing project to develop and characterize gene expression profiles during comparative stages of in vivo and in vitro development of swine embryos. Presented here is a 'work-in-progress' description of current SAGE analyses in swine embryos (and another model system in the lab) to provide an illustrative overview of the major theoretical, technical and bioinformatics issues encountered when applying SAGE to embryos and livestock species.