GENETIC, PHYSICAL, AND INFORMATICS RESOURCES FOR MAIZE: ON THE ROAD TO AN INTEGRATED MAP

Authors: CONE, KAREN - UNIV OF MISSOURI; McMullen, Michael; BI, IRIE - UNIV OF MISSOURI; DAVIS, GEORGIA - UNIV OF MISSOURI; YIM, YOUNG - UNIV OF MISSOURI; GARDINER, JACK - UNIV OF MISSOURI; Schaeffer, Mary; SANCHEZ-VILLEDA, HECTOR - UNIV OF MISSOURI; FANG, ZHIWEI - UNIV OF MISSOURI; SCHROEDER, STEVEN - UNIV OF MISSOURI; HAVERMAN, SETH - UNIV OF MISSOURI; BOWERS, JOHN - UNIV OF GEORGIA-ATHENS; PATERSON, ANDREW - UNIV OF GEORGIA-ATHENS; SODERLUND, CAROL - AZ GENOMICS COMP LAB; ENGLER, FRED - AZ GENOMICS COMP LAB; WING, ROD - UNIV OF ARIZONA; Coe Jr, Edward

Submitted to: Plant Physiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/4/2002
Publication Date: 12/1/2002
Citation: CONE, K., MCMULLEN, M.D., BI, I.V., DAVIS, G.L., YIM, Y.S., GARDINER, J.M., POLACCO, M.L., SANCHEZ-VILLEDA, H., FANG, Z., SCHROEDER, S.G., HAVERMAN, S.A., BOWERS, J.E., PATERSON, A.H., SODERLUND, C.A., ENGLER, F.W., WING, R.A., COE JR, E.H. GENETIC, PHYSICAL, AND INFORMATICS RESOURCES FOR MAIZE: ON THE ROAD TO AN INTEGRATED MAP. PLANT PHYSIOLOGY. 2002. p. 1598-1605.

Interpretive Summary: Crop plant research is poised to make revolutionary strides including: cloning target genes based on their function and/or their position in the genome; documenting all genes and their interplay; defining and exploring all the existing genetic diversity in a species; and using functional information and syntenic relationships of genes in closely-related species to extrapolate gene function in crop plants. The challenge, however, is to develop a set of comprehensive and systematic resources to facilitate these research endeavors. Genomic resources in maize will undergird sequencing of the maize genome, and will complement and contribute to research in the cereals, other grasses, and other crop plants. This paper reports on the process of preparation of comprehensive and systematic genome resources for maize. Information from this study will be important to cereal grains research workers in advancing their efforts to use knowledge from genomics in maize improvement and to understand how to use knowledge from each of the cereals in improvement of each, by identification of gene functions comprehensively and by comparative genetic and physical mapping.

Technical Abstract: To develop an integrated genetic and physical map resource for maize, the strategy and progress are presented on a comprehensive approach that includes three core components. The first is a high-resolution genetic map that provides essential genetic anchor points for ordering the physical map and for utilizing comparative information from other smaller-genome plants. The physical map component consists of contigs assembled from clones from three deep-coverage genomic libraries. The third core component is a set of informatics tools designed to analyze, search and display the mapping data. The criteria and standards for integrating the genetic and physical map, and for representing them, are defined in this paper.