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United States Department of Agriculture

Agricultural Research Service

Title: Potential Use of Bulked-Pollen Methodology in Cotton

Authors
item Gutierrez, Osman
item Jenkins, Johnie
item McCarty, Jack
item Bowman, Daryl - NC STATE UNIVERSITY
item Watson, Clarence - MISS STATE UNIVERSITY

Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Abstract Only
Publication Acceptance Date: January 6, 2003
Publication Date: June 1, 2003
Citation: GUTIERREZ, O.A., JENKINS, J.N., MCCARTY JR, J.C., BOWMAN, D.T., WATSON, C.E. POTENTIAL USE OF BULKED-POLLEN METHODOLOGY IN COTTON. CD-ROM. Proceedings Beltwide Cotton Conference. 2003. p. 784.

Technical Abstract: Genetic diversity provides protection against disease or insect epidemics and is the basis for future genetic gain. Genetic variation and genetic relationship between genotypes are important considerations for germplasm improvement. They play an important role in plant breeding programs by influencing the choice of genotypes to cross for the development of new populations. Linkage as well as pleiotropic effects are responsible for the negative correlation in cotton between agronomic and fiber traits. Intermating and random mating have been used successfully to break the linkage between fiber strength and lint yield. The objectives of this study were to evaluate the utility of bulked-pollen methodology in cotton. Pollen from Stoneville 213 glandless (gl) and TM-1 glanded (GL) were mixed at five different ratios. The following ratios 1:13, 1:26, 1:39, 1:52, and 1:65 were developed by dusting pollen from blooms of Stoneville 213 gl and TM-1 GL into a beaker. Emasculated flowers from the Stoneville 213 (gl) were pollinated with each one of the mixed pollen ratios. The percentage of boll set, total number of seeds and number of glandless seeds in each ratio were determined. Results indicated that ratios 1:13 and 1:65 segregate as expected. Ratio 1:26 showed significant Chi square due to a larger number of gl seeds than expected. Ratios 1:39 and 1:52 also did not segregated as expected because of a low number of gl seeds recovered. This result may be due to the limited number of crosses that were set for these ratios. Cotton pollen mixed well and the boll and seed set were excellent. The bulked-pollen method is a good tool that can be used in the development of breeding population in cotton.

Last Modified: 11/28/2014
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