|Balaji, Boovaraghan - PURDUE UNIVERSITY|
|Ohm, Herbert - PURDUE UNIVERSITY|
Submitted to: Barley Yellow Dwarf Disease: Recent Advances and Future Strategies
Publication Type: Abstract Only
Publication Acceptance Date: July 1, 2002
Publication Date: September 1, 2002
Citation: Balaji, B., Bucholtz, D.L., Ohm, H.W., Anderson, J.M. 2002. Real-time rt-pcr quantification of yellow dwarf virus accumulation and defense gene expression. Barley Yellow Dwarf Disease: Recent Advances and Future Strategies. Technical Abstract: Yellow dwarf virus (YDV) is one of the principal diseases of wheat causing considerable losses in world food grain production. This problem is exacerbated by the fact that wheat does not contain very effective YDV resistance or tolerance genes. High levels of YDV resistance have been introgressed by the introduction of a wheatgrass (Thinopyrum intermedium) chromosome into wheat. Using Quantitative Real-time Reverse Transcription PCR (Q-RT-PCR) the pattern of YDV accumulation and Defense gene expression was determined following inoculation of wheatgrass, resistant and susceptible wheat and susceptible oat lines. These analyses determined that Q-RT-PCR was much more sensitive than ELISA and that the level of viral RNA was not always predicted by the amount of coat protein. This technique was able to detect BYDV P-PAV as little as three hours post infection and showed that P-PAV RNA accumulated much earlier and to a higher level than CYDV-RPV. These patterns of virus accumulation were similar in wheat and oats. Analysis of defense gene expression demonstrated that expression of these genes increases in response to the virus in both resistant and susceptible wheat and susceptible oat lines and the induction of these genes is greater in the susceptible wheat line. These data indicate that these genes are not the factors leading to resistance.