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United States Department of Agriculture

Agricultural Research Service

Title: DNA FINGERPRINTING ANALYSIS OF VEGETATIVE COMPATIBILITY GROUPS IN ASPERGILLUS FLAVUS FROM A PEANUT FEILD IN GEORGIA

Authors
item McAlpin, Cesaria
item Wicklow, Donald
item Horn, Bruce

Submitted to: Mycopathologia
Publication Type: Abstract Only
Publication Acceptance Date: October 24, 2001
Publication Date: January 10, 2002
Citation: Mcalpin, C.E., Wicklow, D.T., Horn, B.W. 2002. Dna fingerprinting analysis of vegetative compatibility groups in aspergillus flavus from a peanut feild in georgia. Mycopathologia.

Interpretive Summary: Not required for "Abstract only"

Technical Abstract: The ability of a species specific DNA probe pAF28 to correctly match 75 strains of A. flavus isolated from a peanut field in Georgia with one of 44 distinct VCGs was assessed. Multiple strains belonging to the same VCG typically produced identical DNA fingerprints with the exception of VCG 17 and VCG 24 which contained strains that showed 83 and 87% similarity, respectively. Aspergillus flavus isolates sharing more that 80% of the fragments are recognized as belonging to the same DNA fingerprint group. Each VCG represented by a single isolate produced unique DNA fingerprints. The results provide further evidence that the pAF28 probe is able to distinguish A. flavus VCGs based on DNA fingerprints and can be used to predict the approximate number of VCGs in a sample population. The DNA probe also hybridized strongly and displayed multiple and distinct bands with other species in Aspergillus section Flavi: A. bombycis, A. caelatus, A. nomius, A. pseudotamarii and A. tamarii. While individual strains representing Aspergillus species in section Flavi produced DNA fragments with multiple bands, the banding patterns could not be used to classify these strains according to species.

Last Modified: 10/31/2014
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