ARS | Publication request: EFFECTS OF RECOMBINANAT IL-6 ON THE FUNCTIONAL ACTIVITIES OF DAY-OLD CHICKEN HETEROPHILS

Submitted to: World Veterinary Poultry Association
Publication Type: Abstract Only
Publication Acceptance Date: July 21, 2003
Publication Date: N/A

Technical Abstract: Previous studies have demonstrated invasion by Salmonella enteritidis (SE) results in an influx of heterophils to the site of infection. Other studies have shown invasion by SE causes an eight- to tenfold increase in the production of the proinflammatory cytokine IL-6. The objective of the present study was to examine the in vitro effects of recombinant interleukin 6 (rIL-6) on the functional activity of day-old chicken heterophils. Heterophils were isolated from day-old leghorn chicks and subsequently incubated with different concentrations of rIL-6 (Cos cell derived) or mock transfected Cos cell supernatant for 2 hours at 39 deg C. Functional activity was assessed using four different assays measuring phagocytosis, oxidative burst, bactericidal killing, and degranulation. Heterophils treated with rIL-6 showed no difference from heterophils that were either untreated or treated with mock transfected Cos cell supernatant. These data would indicate rIL-6, alone, does not increase phagocytic, oxidative, or nonoxidative killing activities of day-old chicken heterophils.