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United States Department of Agriculture

Agricultural Research Service

Title: Phylogenetic Relationships Between the CA 2002 and 1996-2000 Mexican Newcastle Disease Virus Isolates and Other Highly Virulent Ndv Isolates

Authors
item Pedersen, Janice - DVL -NVSL - APHIS
item Senne, Dennis - DVL - NVSL - APHIS
item Woolcock, Peter - UNIV OF CA - DAVIS
item Seal, Bruce

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 2003
Publication Date: July 15, 2003
Citation: Pedersen, J.C., Senne, D.A., Woolcock, P., Seal, B.S. 2003. Phylogenetic relationships between the ca 2002 and 1996-2000 mexican newcastle disease virus isolates and other highly virulent ndv isolates. American Association of Avian Pathologists.

Technical Abstract: Phylogentic analysis was conducted on the Newcastle disease viruses (NDV (avian paramyxovirus type 1 {APMV-1} isolated from game fowl during the 2002 NDV outbreak in southern CA. The NDV isolates have been characterized as highly virulent NDV by amino acid sequence analysis of the fusion gene cleavage site and intracerebral chicken pathogenicity testing. The CA isolates have a diabasic amino acid motif at the fusion protein cleavage site, a requirement for host systemic replication. Phylogentic relationships based on the fusion gene nucleotide sequence were determined for several 1996-2000 Mexican ND viruses as well as the 1998 and 1972 CA and 2000 Honduras ND viruses. Fusion gene sequence analysis revealed a 90% identity between the 2002 CA NDV and Pheasant/1208/CA/98 and a 93% identity with Honduras/15/2000. However, the 2002 CA NDV was 98-99% identical to the Mexican 98 and 2000 NDV. Analysis point to smuggled psittacine bird ND viruses isolated in 1989 as the possible origin of these closely related virulent ND viruses. Amino acid alignment analysis indicate that the CA 2002 and Mexican 2000 viruses have a D for N substitution at position 163 and a N for D substitution at position 170 of the fusion protein. These substitutions were not present in the other viruses compared in this study.

Last Modified: 10/23/2014
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