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United States Department of Agriculture

Agricultural Research Service

Title: Pathogenicity of a Molecular Clone of a Field Strain of Subgroup J Avian Leukosis Virus

Authors
item Lupiani, Blanca - USDA-ARS-MWA-ADOL
item Pandiri, Arun - MICHIGAN STATE UNIVERSITY
item Mays, Jody
item Conklin, Kathleen - UNIV OF MINNESOTA
item Silva, Robert
item Reed, Willie - MICHIGAN STATE UNIVERSITY
item Fadly, Aly

Submitted to: American Veterinary Medical Association Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 19, 2003
Publication Date: July 31, 2003
Citation: Lupiani, B.M., Pandiri, A.R., Mays, J.K., Conklin, K.F., Silva, R.F., Reed, W.M., Fadly, A.M. 2003. Pathogenicity of a molecular clone of a field strain of subgroup J avian leukosis virus [abstract]. American Veterinary Medical Association. p. 100.

Technical Abstract: Using standard methods, ADOL-R5-4, a field strain of subgroup J avian leukosis virus (ALV-J) was molecularly cloned. This molecular clone named ALV-J p-R5-4 was propagated and titrated in line 0 chicken-embryo fibroblasts. The pathogenicity of this molecular clone, ALV-J p-R5-4 was tested in meat-type chickens; day-old chicks were each inoculated with 100,000 infectious units of virus at hatch. Inoculated chickens tested positive for ALV-J viremia and developed tumors within 30 weeks of age. Data indicate that the ALV-J pR5-4 is infectious; it is currently being used to determine role of E element in pathogenesis of the disease.

Last Modified: 11/21/2014
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