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United States Department of Agriculture

Agricultural Research Service

Title: Presence of Campylobacter Jejuni in Various Body Organs on Hour, One Day, One Week Following Oral Or Intracloacal Inoculation of Broiler Chicks

Authors
item Cox, Nelson
item Hiett, Kelli
item Hofacre, C - UGA AVIAN MED
item Bailey, Joseph
item Buhr, Richard
item Wilson, J - UGA POULTRY SCI

Submitted to: Campylobacter Helicobacter and Related Organisms International Workshop
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 2003
Publication Date: August 4, 2003
Citation: Cox, Jr., N.A., Hiett, K.L., Hofacre, C.L., Bailey, J.S., Buhr, R.J., Wilson, J.L. 2003. Presence of Campylobacter jejuni in various body organs on hour, one day, one week following oral or intracloacal inoculation of broiler chicks [Abstract]. Campylobacter Helicobacter and Related Organisms International Workshop. 293:(35):18.

Technical Abstract: Day old broiler chicks were inoculated (either orally or intracloacally) with 106- 109 cells of a characterized strain of Campylobacter jejuni (Cj). At one hour, one day (24h) and one week after inoculation, birds from each group were humanely sacrificed and the bursa, ceca, liver/gallbladder, spleen and thymus were aseptically removed and individually analyzed for Cj. One hour and one day after oral inoculation, Cj was isolated at least once from each of the five sample sites and from all sample sites except the liver/gallbladder, one-week post inoculation. Overall, Cj was isolated from 6/50, 6/50 and 14/45 of the 1h, one-day and one-week samples, respectively, and from 15/29, 1/29, 2/29, 0/29 and 8/29 of the ceca, spleen, thymus, liver/gallbladder and bursa samples, respectively. Following the intracloacal route (IC) of inoculation, Cj was recovered from 17/50, 11/50 and 8/50 of the one hour, one day, and one week samples, respectively, and from 13/30, 1/30, 0/30, 0/30 and 23/30 of the ceca, spleen, thymus, liver/gallbladder and bursa samples, respectively. The next stage will be to introduce Cj and other bacteria to day old breeder chicks and study the carrier state and development of reservoirs throughout their 65-week life cycle. This will allow us to determine if bacteria from these internal body organs result in breeder and ultimately broiler flock contamination.

Last Modified: 7/31/2014
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