Submitted to: American Society of Plant Biologists Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: February 15, 2003
Publication Date: February 15, 2003
Citation: OLIVER, M.J., HUDGEONS, J.L., PAYTON, P.R. MICROARRAY ANALYSIS OF GENE EXPRESSION DURING DESICCATION AND REHYDRATION OF TORTULA RURALIS.. AMERICAN SOCIETY OF PLANT BIOLOGISTS ANNUAL MEETING. Technical Abstract: Gene expression studies of desiccation-tolerant plants are limited to only a few species one of which is the desiccation-tolerant moss Tortula ruralis. From an evolutionary standpoint T. ruralis represents the primitive mechanism for tolerance of dehydration and thus an understanding of its genome level response has important implications for plant biology and agriculture. We have demonstrated that the alteration in gene expression in response to desiccation in this plant occurs following rehydration, in large part as the result of a change in translational controls. In addition, during drying certain transcripts, encoding rehydrins, are sequestered in messenger ribonucleoprotein particles (mRNPs) for storage in the dried state. To determine the extent and importance of translational versus transcriptional events we have taken two approaches. We have established an EST collection, 10,000 cDNA clones, from a rehydration specific library and have constructed rehydration and drying specific Subtractive Suppression Hybridization (SSH) cDNA collections. A small subset of sequenced mostly novel SSH clones, 768 (384 from rehydration and 384 from mRNP collections), has been used to initiate a microarray-based study for gene expression profiling during the desiccation/rehydration response in Tortula. Using both total and polysomal mRNA transcripts for the source of cDNA probes we have evaluated the relative importance of transcript abundance and recruitment in the response for each represented gene. The EST collection has been used to develop a unigene array that is also being evaluated in a similar fashion. The data we will present offers a unique picture of the transcriptome (and "translatome") level response of T. ruralis to severe stress.