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United States Department of Agriculture

Agricultural Research Service

Title: High-Throughput Screening of Enrofloxacin in Chicken Muscle by Spectrofluorimetry

Authors
item CHEN, GUOYING
item SCHNEIDER, MARILYN

Submitted to: Feedinfo News Service
Publication Type: Other
Publication Acceptance Date: September 10, 2003
Publication Date: September 11, 2003
Citation: CHEN, G., SCHNEIDER, M.J. HIGH-THROUGHPUT SCREENING OF ENROFLOXACIN IN CHICKEN MUSCLE BY SPECTROFLUORIMETRY. FeedInfo News Service. 2003.

Technical Abstract: A simple and rapid spectrofluorimetric method was developed for screening enrofloxacin (ENRO) in chicken muscle in which a simple single-step extraction in acidic acetonitrile was performed without further clean up. Following centrifugation the supernatants were excited at 324 nm and the emission measured at 442 nm. Eighteen chicken breast samples from three producers were tested and the results showed background signal levels below 300 ug/kg, the FDA approved tolerance level. The calibration curve revealed a satisfactory linear relationship (R(2) = 0.9991) over a range of 0 - 700 ug/kg in ENRO fortified chicken breast. ENRO incurred samples were examined using this approach, and the results agreed well with those obtained from more extensive separation followed by high-performance liquid chromatography. The method proved reproducible. Screening of ENRO at the tolerance level can be accomplished with a threshold limit set at 3 sigma. Based on this investigation, a high-throughput screening method for ENRO in chicken tissue is proposed.

Last Modified: 9/10/2014
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