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United States Department of Agriculture

Agricultural Research Service

Title: A Good Endophyte of Maize: Acremonium Zeae Antibiotics Inhibitory to Aspergillus Flavus and Fusarium Verticillioides

Authors
item Wicklow, Donald
item Roth, Shoshannah - U OF IOWA, IOWA CITY
item Deyrup, Stephen - U OF IOWA, IOWA CITY
item Gloer, James - U OF IOWA, IOWA CITY

Submitted to: Aflatoxin Workshop
Publication Type: Abstract Only
Publication Acceptance Date: October 15, 2003
Publication Date: N/A

Technical Abstract: The maize endophyte Acremonium zeae Gams and Sumner is antagonistic to kernel rotting and mycotoxin producing fungi Aspergillus flavus and Fusarium verticillioides in cultural tests for antagonism and interferes with A. flavus infection and aflatoxin contamination of preharvest maize kernels. Chemical studies of an organic extract from maize kernel fermentations of A. zeae NRRL 13540, which displayed significant antifungal activity against A. flavus and F. verticillioides, revealed that the metabolites accounting for this activity were two newly reported antibiotics pyrrocidines A and B. Pyrrocidines were detected in fermentation extracts for nine NRRL cultures of A. zeae isolated from maize kernels harvested in Illinois (3/3 cultures), North Carolina (5/5) and 'USA' (1/1). Pyrrocidine B was detected by LCMSMS in whole symptomatic maize kernels removed at harvest from ears of a commercial hybrid that were wound-inoculated in the milk stage with A. zeae NRRL 13540 or NRRL 13541. The pyrrocidines were first reported from the fermentation broth of an unidentified filamentous fungus LL-Cyan-426, isolated from a mixed Douglas Fir hardwood forest on Crane Island Preserve, Washington, in 1993 [H. He et al., Tetrahedron Letters 43 (2002) 1633-1636]. It was reported that pyrrocidine A exhibits potent activity against most Gram-positive bacteria, including drug-resistant strains, and was also active against the yeast Candida albicans. In an evaluation of cultural antagonism between isolates of A. zeae in pairings with A. flavus NRRL 6541 and F. verticillioides NRRL 25457, A. zeae NRRL 6415 produced the strongest reaction, inhibiting both organisms at a distance while continuing to grow through the resulting clear zone at an unchanged rate. Maximum colony diameters for A. zeae NRRL 6415 and NRRL 13540, on potato dextrose agar after 14 days, were attained within the range of 25-30 C, with less growth recorded at 15 C and 37.5 C and no growth at 5 C. Potential interactions between A. zeae and other maize endophytes are considered and the significance of these interactions relative to the aflatoxin and fumonisin contamination of preharvest maize is presented. This is the first report of natural products from Acremonium zeae.

Last Modified: 8/2/2014
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