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United States Department of Agriculture

Agricultural Research Service

Title: Efficacy of Boiling Water Immersion for Collection of Separate External and Internal Microbiological Samples of Breeder Testes for Salmonella and Campylobacter Spp

item Buhr, Richard
item Bailey, Joseph
item Cosby, Douglas
item Bourassa, Dianna
item Richardson, Larry
item Musgrove, Michael

Submitted to: Poultry Science
Publication Type: Abstract Only
Publication Acceptance Date: May 4, 2003
Publication Date: July 6, 2003
Citation: Buhr, R.J., Bailey, J.S., Cosby, D.E., Bourassa, D.V., Richardson, L.J., Musgrove, M.T. 2003. Efficacy of boiling water immersion for collection of separate external and internal microbiological samples of breeder testes for salmonella and campylobacter spp. [abstract] Poultry Science. 82(suppl.1):6.

Technical Abstract: To determine if hot water immersion would kill bacteria on the external surface of the testes, we aseptically collected 60 testes from broiler-breeder roosters that were partially processed, prior to evisceration. Testes were placed into Stomacher 400 bags and transported to the laboratory on ice where the testes were placed into one of nine groups (five testes per group). One group served as the negative control and was not inoculated or immersed in hot water. Four groups were inoculated with a cocktail of Nalidixic Acid resistant Salmonella (Sal NR), two internally and two externally. The remaining four groups were inoculated with Campylobacter (Campy), two internally and two externally. Testes of the control group underwent standard FSIS testing for Sal NR and Campy. For half of the Sal and Campy inoculated groups, individual testes were placed into 100 ml sterile plastic disposable specimen cups. Enough boiling water was added to the testes in order to cover the testes and allowed to remain for 30 s. The water was decanted, the testes placed into bags and placed into an ice bath. The remaining groups were not subjected to boiling water and served as positive external and internal inoculated controls. The testis was smashed with a mallet, BPW added, and the suspension serially diluted onto BGS agar plates with nalidixic acid (Sal NR) or Campy-CEFEX (Campy) plates. No positives colonies were detected in the negative control group testes. Recovery levels did not differ significantly between the internal inoculated-boiled testes, internal inoculated-not boiled testes, and the external inoculated-not boiled testes. There was a four log reduction in numbers of Sal NR for the external inoculated-boiled testes compared to the external inoculated-not boiled testes, and a corresponding three log reduction for Campy. With the low numbers of bacteria typically recovered from the external surface of a healthy rooster's testes, this boiling method provides a way to sample the internal tissue of the testes and eliminate potential for air sac contamination of the sample.

Last Modified: 4/22/2015
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