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Title: ALTERNANSUCRASE ACCEPTOR REACTIONS WITH D-TAGATOSE AND L-GLUCOSE

Author
item Cote, Gregory
item Dunlap, Christopher

Submitted to: American Chemical Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 4/2/2004
Publication Date: 4/2/2004
Citation: Cote, G.L., Dunlap, C.A. 2004. Alternansucrase acceptor reactions with D-tagatose and L-glucose [abstract]. American Chemical Society. Paper No. 35.

Interpretive Summary:

Technical Abstract: Alternansucrase (EC 2.4.1.140) is a D-glucansucrase that synthesizes an alternating alpha-(1-3), (1-6)-linked D-glucan from sucrose. In the presence of D-tagatose, alternansucrase produced the disaccharide alpha-D-glucopyranosyl-(1-1)-beta-D-tagatopyranose (I) via glucosyl transfer. This disaccharide is analogous to trehalulose. We were unable to isolate a disaccharide product from L-glucose, but the trisaccharide alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl-(1-4)-L-glucose (II) was isolated and identified. This is analogous to panose, one of the structural units of pullulan, in which the reducing-end D-glucose residue has been replaced by its L-enantiomer.