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United States Department of Agriculture

Agricultural Research Service

Title: Seven New Ovine Progressive Pneumonia Virus (Oppv) Field Isolates from Dubois Idaho Sheep Comprise Part of Oppv Clade II Based on Surface Envelope Glycoprotein (Su) Sequences

Authors
item Hoesing, Lynn
item Hotzel, I - WASHINGTON STATE UNIV
item Cheevers, W - WASHINGTON STATE UNIV
item Pretty On Top, K - WASHINGTON STATE UNIV
item Lewis, Gregory
item Knowles, Donald

Submitted to: Virus Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: February 3, 2004
Publication Date: February 4, 2004
Citation: Herrmann, L.M., Hotzel, I., Cheevers, W.P., Pretty On Top, K., Lewis, G.S., Knowles, D.P. SEVEN NEW OVINE PROGRESSIVE PNEUMONIA VIRUS (OPPV) FIELD ISOLATES FROM DUBOIS IDAHO SHEEP COMPRISE PART OF OPPV CLADE II BASED ON SURFACE ENVELOPE GLYCOPROTEIN (SU) SEQUENCES. Virus Research. 2004. v. 102. p. 215-220.

Interpretive Summary: Ovine progressive pneumonia virus (OPPV) can cause severe pneumonia leading to death in some sheep flocks. Seven new OPPV field isolates were derived from colostrum and milk of 10 naturally OPPV-infected sheep from the U.S. Sheep Experiment Station in Dubois, Idaho, USA. These new OPPV field isolates are more closely related to other OPPV isolates in terms of nucleotide sequence and amino acid sequence of the surface envelope glycoprotein. Twenty-four years has past without new information regarding OPPVs in the field. This report provides new information regarding present day OPPV field isolates in the western U.S.

Technical Abstract: Seven new ovine progressive pneumonia virus (OPPV) field isolates were derived from colostrum and milk of 10 naturally OPPV-infected sheep from the U.S. Sheep Experiment Station in Dubois, Idaho, USA. Sixteen sequences of the surface envelope glycoprotein (SU) from these 7 Dubois OPPV field isolates and SU sequence from OPPV WLC1 were obtained, aligned with published SRLV SU sequences, and analyzed using Phylogenetic Analysis Using Parsimony (PAUP). Percent nucleotide identity in SU was greater than 95.8% among clones from individual Dubois OPPVs and ranged from 85.5-93.8% between different Dubois OPPV clones. SU sequences from Dubois OPPVs and WLC1 OPPV had significantly higher percent nucleotide identity to SU sequences from the North American OPPVs (85/34 and S93) than CAEVs or MVVs. PAUP analysis also showed that SU sequences from the Dubois OPPVs and OPPV WLC1 grouped with other North American OPPVs (85/34 and S93) with a bootstrap value of 100 and formed one OPPV clade II group. In addition, Dubois and WLC1 SU amino acid sequences had significantly higher identity to SU sequences from North American OPPVs than CAEV or MVV. These data indicate that the 7 new Dubois OPPV field isolates along with WLC1 OPPV are part of the OPPV clade II and are distinct from CAEVs and MVVs.

Last Modified: 10/1/2014
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