Submitted to: Conference Research Workers Disease Meeting
Publication Type: Abstract Only
Publication Acceptance Date: November 9, 2003
Publication Date: November 9, 2003
Citation: Harp, J.A., Stabel, J.R., Pesch, B.A., Goff, J.P. 2003. Comparison of adhesion molecule expression on lymphocytes from blood and milk of normal cows and cows with Johne's disease [abstract]. Conference Research Workers Disease Meeting. p. 71P. Technical Abstract: Fifteen normal dairy cows and 12 dairy cows with Johne's disease were monitored for expression of adhesion molecules on lymphocytes in blood and milk at parturition and at intervals up to 21 days postpartum. Using flow cytometric analysis, we examined expression of CD62L, CD11a and alpha4/beta7 on T cell subsets (CD4+, CD8+, gamma/delta+). CD62L was expressed on 20-70% of T cell subsets in blood and 35-80% in milk from normal cows. In cows with Johne's disease the range was 50-80% and 75-100%, respectively. CD11a was found in 10-25% of T cells in blood and 35-70% in milk from normal cows, compared to 10-40% and 20-75%, respectively, in cows with Johne's disease. Alpha4/beta7 was expressed on 5-10% of T cells in blood and 10-35% in milk from normal cows. The range was 10-30% in blood and 75-100% in milk from cows with Johne's disease. These differences, especially those seen in CD62L and alpha4/beta7 on milk lymphocytes, suggest that infection with Johne's disease affects adhesion molecule expression and subsequent recruitment into the bovine mammary gland. Since CD62L and alpha4/beta7 are peripheral and mucosal homing receptors, respectively, these data suggest altered recruitment of both pools of recirculating lymphocytes into the mammary gland of cows with Johne's disease.