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United States Department of Agriculture

Agricultural Research Service

Title: Identifying Limonoid Aglycones by Electron Ionization and Atmospheric Pressure Chemical Ionization Liquid Chromatography - Mass Spectrometry Techniques

item Manners, Gary
item Breksa, Andrew

Submitted to: Phytochemical Analysis
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 26, 2004
Publication Date: December 1, 2004
Citation: Manners, G.D., Breksa III, A.P. 2004. Identifying Citrus Limonoid Aglycones by HPLC-EI/MS and HPLC-APCI/MS Techniques. Phytochemical Analysis. V. 15:372-381.

Interpretive Summary: A chromatographic method that efficiently separates citrus limonoids commonly found in citrus fruits and juices has been coupled with two different mass spectrometric detection methods (electron ionization, atmospheric chemical ionization) to gather chemical information about the 17 known limonoids occurring in citrus. The liquid chromatography-mass spectrometry information obtained from the known limonoids can be used to postulate chemical structural information to aid in identifying unknown limonoids found in citrus materials.

Technical Abstract: Normal phase electron ionization (EI) and atmospheric chemical ionization liquid chromatography (APCI) - mass spectrometry methods are applied to 17 known neutral limonoid aglycones from citrus sources. The liquid chromatography-mass spectrometry data from the known limonoids provides chromatographic characteristics, APCI derived molecular weight data and mass spectral EI fragmentation data that can be utilized to postulate the structural character of unknown citrus limonoids.

Last Modified: 4/22/2015
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