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United States Department of Agriculture

Agricultural Research Service

Title: Evaluation of the Humoral Immune Response to Ibdv in Specific-Pathogenic-Free Chickens Using Flow Cytometry Analysis and Elisa

Authors
item Petlov, Daniel - UNIV OF GEORGIA-ATHENS
item Linnemann, Erich - UNIV OF GEORGIA-ATHENS
item Kapczynski, Darrell
item Sellers, Holly - UNIV OF GEORGIA-ATHENS

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: May 23, 2004
Publication Date: July 28, 2004
Citation: Petlov, D.I., Linnemann, E.G., Kapczynski, D.R., Sellers, H.S. 2004. Evaluation of the humoral immune response to ibdv in specific-pathogenic-free chickens using flow cytometry analysis and elisa. In:Proceedings of the American Association of Avian Pathologists, July 24-28, 2004, Philadelphia, Pennsylvania. CDROM.

Technical Abstract: Infectious bursal disease virus (IBDV) is an immunosuppressive virus which targets chicken IgM+, B-cells. It is speculated that these cells are immature IgM+, B-cells. Chickens were vaccinated at 3 days of age with an intermediate classical vaccine and challenged at 22 days of age with the Edgar strain. Bursa and spleen samples were analyzed by Flow cytometry and ELISA. Flow cytometry analysis of bursa samples showed two IgM+, B-cell subpopulations: A and B. The only reduction of B-cells was observed in IgM+, B-cell, subpopulation B. Reduction of IgG+, IgA+, B-cells in bursa and spleen was not observed. IgA+, B-cells in bursa and IgM+, B-cells in spleen increased 14 days post-infection. Levels of total serum IgM, IgA IgG, and anti-IBDV immunoglobulin will be evaluated.

Last Modified: 10/1/2014
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