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United States Department of Agriculture

Agricultural Research Service

Title: Effect of Insulin and Growth Hormone Administration to Mature Miniature Brahman Cattle on Circulating Concentrations of Metabolic Hormones and Metabolites

Authors
item Chase, Chadwick
item Riley, David
item Elsasser, Theodore
item Spicer, L. - OKLAHOMA STATE UNIV.
item Lucy, M. - UNIV. OF MISSOURI
item Coleman, Samuel
item Olson, T. - UNIV. OF FLORIDA

Submitted to: Journal of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: April 2, 2004
Publication Date: June 10, 2004
Citation: Chase, C.C., Riley, D.G., Elsasser, T.H., Spicer, L.J., Lucy, M.C., Coleman, S.W., Olson, T.A. 2004. Effect of insulin and growth hormone administration to mature miniature brahman cattle on circulating concentrations of metabolic hormones and metabolites. Journal of Animal Science: Vol.82, Suppl.1/ Journal of Dairy Science: Vol.87, Suppl.1/ Poult. Sci. Vol. 83, Suppl.1.

Technical Abstract: The objective of this study was to determine the effect of administration of GH, insulin (INS), and GH plus INS to mature miniature Brahman cows (n = 6; 9.7 ' 2.06 yr; 391 ' 48.6 kg) and bulls (n = 8; 9.4 ' 2.00 yr; 441 ' 54.0 kg) on plasma concentrations of metabolic hormones (GH, INS, IGF-I) and metabolites (glucose, urea nitrogen [PUN]). We hypothesized that IGF-I secretion could be enhanced by concomitant administration of exogenous GH and INS, but not by either hormone alone. Animals were allotted to a modified crossover design that included four treatments: control (CON), GH, insulin (INS), and GH+INS. At the start of the study, one-half of the animals were administered GH (POSILAC; 14-d slow release) and the other one-half served as controls (CON) for 7 d. Beginning on day 8 and for 7 d, insulin (Novolin L) was administered (0.125 IU/kg BW) twice daily (0700 and 1900) to all animals; hence the INS and GH+INS treatments. Animals were rested for 14 d and then were switched to the other treatment combination. Blood samples were collected at 12-h intervals during the study. Data were analyzed using PROC MIXED and fixed effects included age, sex, treatment, period, and interactions. Animal within treatment-period combinations was a random effect. Sex affected (P < 0.05) plasma concentrations of metabolic hormones but not (P > 0.15) blood metabolites. Compared to CON, GH treatment increased (P < 0.01) mean plasma concentrations of GH (11.1 vs 15.7 ' 0.94 ng/mL), INS (0.48 vs 1.00 ' 0.081 ng/mL), IGF-I (191.3 vs 319.3 ' 29.59 ng/mL), and glucose (73.9 vs 83.4 ' 2.12 mg/dL), but decreased (P < 0.05) PUN (14.2 vs 11.5 ' 0.75 mg/dL). Compared to INS, GH+INS treatment increased (P < 0.05) mean plasma concentrations of INS (0.71 vs 0.96 ' 0.081 ng/mL), IGF-I (228.7 vs 392.3 ' 29.74 ng/mL) and glucose (48.1 vs 66.70 ' 2.12 mg/dL), decreased (P < 0.01) PUN (13.6 vs 10.4 ' 0.76 mg/dL), and did not affect GH (13.5 vs 12.7 ' 0.95 ng/mL). In the miniature Brahman model, using mature animals, both GH and GH+INS treatments dramatically increased circulating concentrations of IGF-I.

Last Modified: 10/23/2014
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