Technical Abstract: We have mapped 2148 loci representing 919 expressed sequence tag (EST) clones into chromosomes of group 7 of wheat. Regional localization and distribution of these markers directly reflect distribution of gene-rich/poor areas in chromosomes of this group. The individual chromosome deletion maps showed that the average number of loci per unit recombination length in the distal two-third regions of all chromosomes varied from 3 to 6 as compared with proximal regions having ratio of 0.3 to 1. Level of duplicated loci on this group was 55% with more of these loci localized towards the distal region. Expressed sequence tag clones producing three bands on EcoRI-digested DNA blots, not duplicated anywhere else in the genome, and mapping to the three homoeologous chromosomes of group 7, were considered as landmark probes. A consensus chromosome map of group 7 was constructed with 119 landmarks probes. Putative order of these probes in the deletion bins was identified based on corresponding rice BACs/PACs and genetic markers mapped on chromosomes 6 and 8 of rice. Comparative maps of wheat, rice, and barley were produced based on the ordered sequences. Identification of landmarks ESTs and the consensus map provides a framework of conserved coding regions predating the evolution of wheat genomes.