Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: April 15, 2004
Publication Date: June 20, 2004
Citation: Luster, D.G., McMahon, M.B., Choi, J.J., Carter, M.L., Nunez, A., Frederick, R.D. 2004. Identification of proteins expressed during resistant and susceptible soybean-rust interactions. Phytopathology. 94:S64. Technical Abstract: Soybean rust, caused by Phakopsora pachyrhizi, is a devastating disease that has spread from Asia to Africa and South America, but not yet to the continental United States. We focused on proteins involved in early responses between P. pachyrhizi and soybean (Glycine max). Proteins localized to the leaf apoplast were extracted from soybean cultivars following inoculation with isolates of soybean rust that resulted in susceptible and resistant interactions. Samples were taken at 0, 12, 24, 48, 72 hours and 6 days post inoculation. Trypsin-digested two-dimensional gel electrophoresis spots were peptide finger-printed and sequenced using Matrix-Assisted Laser Desorption/Ionization mass spectrometry with automated tandem time of flight fragmentation of selected ions (MALDI-TOF/TOF). Proteins identified by database searches from putative peptide sequences within a 95% confidence interval were evaluated and placed into functional categories. Results show a large group of proteins involved in defense response and cell signaling.