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Title: INDUCED SYSTEMIC RESISTANCE (ISR) IN ARABIDOPSIS AGAINST PSEUDOMONAS SYRINGAE PV. TOMATO BY 2,4-DIACETYLPHLOROGLUCINOL (DAPG)-PRODUCING PSEUDOMONAS FLUORESCENS.

Author
item Weller, David
item VAN PELT, J - UNIVERSITEIT UTRECHT
item MAVRODI, D - WASHINGTON STATE UNIV
item PIETERSE, C - UNIVERSITEIT UTRECHT
item BAKKER, P.A. - UNIVERSITEIT UTRECHT
item VAN LOON, L - UNIVERSITEIT UTRECHT

Submitted to: APS Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/20/2004
Publication Date: 7/20/2004
Citation: Weller, D.M., Van Pelt, J.A., Mavrodi, D.V., Pieterse, C.M., Bakker, P.H., Van Loon, L.C. 2004. Induced systemic resistance (isr) in arabidopsis against pseudomonas syringae pv. tomato by 2,4-diacetylphloroglucinol (dapg)-producing pseudomonas fluorescens. APS Annual Meeting. Phytopathology 94: S108.

Interpretive Summary:

Technical Abstract: Two-week-old Arabidopsis thaliana ecotype Col-0 seedlings were transferred into an autoclaved sand-soil mixture colonized by DAPG-producing P. fluorescens strain Pf-5 (rep-PCR genotype A), Q2-87 (B), Q8r1-96 (D) or HT5-1 (N) and the plants were challenge-inoculated with Pseudomonas syringae three weeks later. DAPG producers were not detected on the leaves. All DAPG producers induced resistance against P. syringae equivalent to the well studied Dutch strain P. fluorescens 417r. phlD or phlBC mutants of Q2-87 (DAPG minus) were significantly reduced in ISR activity. A phlF regulatory mutant (over-producer) had ISR activity equivalent to Q2-87. Introduction of DAPG into soil at concentrations of 10 to 250 'M four days before challenge inoculation induced resistance equivalent to or better than the bacteria. These results suggest that DAPG is a major mechanism of ISR by DAPG producers.