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Title: BACTERIOCIN ACTIVITY AMONG 2,4-DIACETYLPHLOROGLUCINOL (DAPG)-PRODUCING FLUORESCENT PSEUDOMONAS SPP.

Author
item MAVRODI, D - WASHINGTON STATE UNIV
item VALIDOV, S - INST. OF BIOCHEMISTRY, RU
item DE LA FUENTE, L - WASHINGTON STATE UNIV.
item BORONIN, A - INST. OF BIOCHEMISTRY, RU
item Weller, David
item Thomashow, Linda

Submitted to: APS Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/20/2004
Publication Date: 7/20/2004
Citation: Mavrodi, D., Validov, S., De La Fuente, L., Boronin, A., Weller, D.M., Thomashow, L.S. 2004. Bacteriocin activity among 2,4-diacetylphloroglucinol (dapg)-producing fluorescent pseudomonas spp. APS Annual Meeting. Phytopathology 94: S67.

Interpretive Summary:

Technical Abstract: Certain DAPG-producing P. fluorescens strains colonize roots and suppress soilborne pathogens more effectively than others from which they are almost indistinguishable. We recovered DNA fragments from the superior colonizer Q8r1-96 by suppression subtractive hybridization with DNA from the moderate colonizer Q2-87 in order to identify genetic differences that might account for superior colonization activity. One Q8r1-96 clone exhibited similarity to a pore-forming bacteriocin and resides in a 23-kb pyocin-like cluster that includes a functional two-gene lysis module and bacteriophage tail assembly. Treatment of Q8r1-96 with mitomycin C caused production of phage tail-like particles. Subsequent screening revealed that bacteriocin activity was common among DAPG-producing isolates representing 17 distinct genotypes and may contribute to strain competitiveness and persistence in vitro and in the rhizosphere.