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United States Department of Agriculture

Agricultural Research Service

Title: Bacteriocin Activity among 2,4-Diacetylphloroglucinol (Dapg)-Producing Fluorescent Pseudomonas Spp.

Authors
item Mavrodi, D - WASHINGTON STATE UNIV
item Validov, S - INST. OF BIOCHEMISTRY, RU
item DE La Fuente, L - WASHINGTON STATE UNIV.
item Boronin, A - INST. OF BIOCHEMISTRY, RU
item Weller, David
item Thomashow, Linda

Submitted to: APS Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: May 20, 2004
Publication Date: July 20, 2004
Citation: Mavrodi, D., Validov, S., De La Fuente, L., Boronin, A., Weller, D.M., Thomashow, L.S. 2004. Bacteriocin activity among 2,4-diacetylphloroglucinol (dapg)-producing fluorescent pseudomonas spp. APS Annual Meeting. Phytopathology 94: S67.

Technical Abstract: Certain DAPG-producing <i>P. fluorescens</i> strains colonize roots and suppress soilborne pathogens more effectively than others from which they are almost indistinguishable. We recovered DNA fragments from the superior colonizer Q8r1-96 by suppression subtractive hybridization with DNA from the moderate colonizer Q2-87 in order to identify genetic differences that might account for superior colonization activity. One Q8r1-96 clone exhibited similarity to a pore-forming bacteriocin and resides in a 23-kb pyocin-like cluster that includes a functional two-gene lysis module and bacteriophage tail assembly. Treatment of Q8r1-96 with mitomycin C caused production of phage tail-like particles. Subsequent screening revealed that bacteriocin activity was common among DAPG-producing isolates representing 17 distinct genotypes and may contribute to strain competitiveness and persistence in vitro and in the rhizosphere.

Last Modified: 11/25/2014
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