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United States Department of Agriculture

Agricultural Research Service

Title: Molecular Heterogeneity of the Chalcone Synthase Intron in Petunia

item Griesbach, Robert
item Beck, Ronald

Submitted to: HortScience
Publication Type: Abstract Only
Publication Acceptance Date: February 2, 2000
Publication Date: May 15, 2000
Citation: Griensbach, R., Beck, R. 2000. Molecular heterogeneity of the chalcone synthase intron in petunia. HortScience. 35:1347-1349

Technical Abstract: In order to study the phylogenetic relationships of closely related species, one should analysis non-coding sequences that have accumulated a wide array of mutations. The intron in the chalcone synthase gene intron is a highly variable sequence that can be easily PCR amplified and sequenced. The sequence of this intron was used to characterize P. integrifolia subsp. integrifolia var. depauperata, P. altiplana, P. littoralis and an unknown taxon from the town of Torres in Brazil. Based upon the intron, the Torres taxon most closely resembled P. integrifolia. The unrooted phylogenetic tree suggested that P. integrifolia was more closely related to P. littoralis than P. altplana.

Last Modified: 4/20/2015
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