Submitted to: Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: May 7, 2004
Publication Date: May 22, 2004
Citation: Bassett, C.L., Farrell, R., Artlip, T.S., Wisniewski, M.E. 2004. Identification of genes expressed in peach bark in response to cold treatment and under different photoperiods. Meeting Proceedings. The 2nd International Rosaceae Genome Mapping Conference, May 2004, pg. 6. Technical Abstract: Living cells respond to environmental signals by up-regulating specific subsets of genes while down-regulating others. Global approaches to identifying these different groups of genes have been successfully applied to several plant systems. However, certain limitations restrict the degree to which each approach is successful in documenting differences in expression. For example, microarray analysis is restricted to previously isolated genes and does not allow identification of unique, undiscovered genes that might be crucial to the response being studied. EST library approaches overcome this problem, but are labor intensive and target for the most part genes that are moderately-to-highly abundant. One approach that overcomes these limitations is the synthesis of gene libraries after subtractive hybridization of cDNAs from different treatments. By varying which cDNA serves as the driver (in excess in the hybridization reaction) and which cDNA serves as the testor, one can obtain both up-regulated (forward subtraction hybridization) and down-regulated (reverse subtraction hybridization) genes in response to a given condition or treatment. To profile gene expression at different temperatures under different photoperiods, we have created subtracted libraries from peach (Prunus persica [L.] Batsch.) bark tissues sampled from trees kept at 5ºC and 25ºC under a short day (SD) photoperiod or exposed to a night break (NB) interruption during the dark period of the SD cycle. Sequences expressed in forward and reverse subtractions using various subtracted combinations of temperature and photoperiod treatments were cloned, sequenced, and identified by BLAST analysis. Genes unique to each treatment and genes that overlap between two or more treatments are presented.