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ARS Home » Plains Area » Houston, Texas » Children's Nutrition Research Center » Research » Publications at this Location » Publication #166257
Title: EFFECTS OF DIETARY PROTEIN ON PROTEIN SYNTHESIS IN NEONATAL PIGS

Author
item FRANK, JASON - BAYLOR COLL OF MEDICINE
item ESCOBAR, JEFFERY - BAYLOR COLL OF MEDICINE
item KIMBALL, SCOT - PENN STATE UNIVERSITY
item SURYAWAN, AGUS - BAYLOR COLL OF MEDICINE
item NGUYEN, HANH - BAYLOR COLL OF MEDICINE
item LIU, CHUN - BAYLOR COLL OF MEDICINE
item JEFFERSON, LEONARD - BAYLOR COLL OF MEDICINE
item Davis, Teresa

Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 3/23/2004
Publication Date: 4/17/2004
Citation: Frank, J.W., Escobar, J., Kimball, S.R., Suryawan, A., Nguyen, H.V., Liu, C.W., Jefferson, L.S., Davis, T.A. 2004. Effects of dietary protein on protein synthesis in neonatal pigs [abstract]. Federation of American Societies for Experimental Biology Conference. 18(4):A377.

Interpretive Summary: Not needed for an Abstract

Technical Abstract: Limited data suggest that growth of low birth weight infants is enhanced by feeding a high protein diet, however the mechanisms involved in the effect have not been delineated. Pigs (N = 18) were fed (60 g dry matter·kg BW-1·d-1) isocaloric milk diets with increased levels of dietary protein (21, 33, and 45% of dry matter) from 2 to 7 d of age. Protein synthesis, various biomarkers of translational regulation, and plasma glucose (GLC) and insulin (INS) were measured in fasted and fed states. Plasma GLC (P < 0.05) and INS (P < 0.10) levels decreased as dietary protein increased. Protein synthesis in longissimus dorsi (LD), liver, and small intestine (SI) was greater in the fed state (P < 0.05). Also, protein kinase B, ribosomal protein S6 kinase, and 4E-BP1 were more phosphorylated and assembly of the inactive eIF4E·4E-BP1 complex was reduced in LD and liver in the fed state (P < 0.05). Protein synthesis in LD decreased linearly with increasing dietary protein in the fasted state, but increased and plateaued at 33% dietary protein in the fed state (P < 0.07). Protein synthesis in SI increased linearly with dietary protein in the fed state only (P < 0.01). Thus, protein synthesis in neonatal pigs is influenced by feeding state and dietary protein level. These changes in part involve modulation of the availability of eIF4E for eIF4F assembly and may be mediated by plasma INS, GLC, and/or amino acid levels.