A PLASMID-BASED FUNCTIONAL PROTEOMIC WORKCELL USED TO OPTIMIZE YEAST FOR FUEL ETHANOL PRODUCTION

Authors: Hughes, Stephen; Li, Xin Liang; Cotta, Michael; Mertens, Jeffrey; Bischoff, Kenneth; RIEDMULLER, STEVEN - HUDSON CONTROL GROUP; FARRELLY, PHIL - HUDSON CONTROL GROUP; PATEL, MEHUL - SIAS, INC.; BROWN, LEE - SIAS, INC.; CARTER, LEE - SIAS, INC.

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 1/14/2005
Publication Date: 1/14/2005
Citation: Hughes, S.R., Li, X., Cotta, M.A., Mertens, J.A., Bischoff, K.M., Riedmuller, S., Farrelly, P., Patel, M., Brown, L., Carter, L. 2005. A plasmid-based functional proteomic workcell used to optimize yeast for fuel ethanol production [abstract]. CHI's Annual PEP Talk Meeting, January 10-14, 2005, San Diego, California. p. 3.

Interpretive Summary:

Technical Abstract: We are developing a high throughput plasmid-based functional proteomic workcell that integrates in a robotic platform the operations of media filling, colony picking, plate sealing, advanced liquid handling, incubation and shaking, and assay data collection into a custom database, with 2D bar code tracking of plates, clones, strains, and datapoints, for rapid optimization of clones and expression of full-length cDNA libraries to produce improved fuel ethanol yeast strains. In producing the plasmid-based libraries of full-length cDNAs that are the source of the optimized sequences for expression in yeast, the robotic platform must be capable of running in vitro and in vivo expression reactions to obtain proteins in proper conformation so that clones having optimal traits can be identified before placement into the yeast strains. Using the same high throughput platform, the yeast strains can be screened for growth characteristics and production of ethanol via standard biochemical assays, and this presentation will discuss the design, construction, and validation of these systems.