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United States Department of Agriculture

Agricultural Research Service

Title: Bran Extracts from Pigmented Rice Seeds Inhibit Phorbol Ester Tumor Promotion in Lymphoblastoid B Cells

Authors
item Choi, Sun Phil - AJOU UNIVERSITY, KOREA
item Kang, Mi Young - KYUNGPOOK NAT.UNIV. KOREA
item Koh, He-Jong - SEOUL NAT.UNIV. KOREA
item Kozukue, Nobvuyuki - YEUNGNAM UNIV. KOREA
item Nam, Seok Hyun - AJOU UNIVERSITY KOREA
item FRIEDMAN, MENDEL

Submitted to: Food and Chemical Toxicology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 25, 2005
Publication Date: March 15, 2005
Citation: Choi, S., Kang, M., Koh, H., Kozukue, N., Nam, S., Friedman, M. 2005. Bran extracts from pigmented rice seeds inhibit phorbol ester tumor promotion in lymphoblastoid b cells. Food and Chemical Toxicology.43:741-745.

Interpretive Summary: As part of an effort designed to discover new, naturally-occurring antimicrobial and antiviral compounds, we participated in a collaborative study carried out in Korea which showed that compared to an extract from the bran of nonpigmented white cooking rice variety Chuchung, the extracts from the pigmented varieties strongly inhibited phorbol ester-induced tumor promotion in marmoset lymphoblastoid cells B95-8 in vitro. The information should be useful for applying the short-term test of anti-tumor-promoting activities of rice bran to other natural products and for evaluating pigmented rice and their biologically active compounds as potnetial antibacterial and antiviral agents against foodborne pathogens.

Technical Abstract: Flow cytometry was used for quantitative evaluation of anti-tumor promoting activity by an in vitro short-term assay system measuring inhibition of Epstein-Barr virus early-antigen activation (EBV-EA) induced by the tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA). This assays simultaneously measures both anti-tumor-promoting activity and cytotoxicity of target substances using the same batch of cells. Using this modified short-term procedure, we determined the anti-tumor promoting activity of 70% ethanol-water extracts of bran (outer layer) from seeds of five pigmented rice cultivars: JumLalocal-1, DZ 78, Elwee, LK1-3-6-12-1-1, and LK1A-2-12-1-1. The results show that compared to an extract from the nonpigmented white cooking rice variety Chuchung, the extracts from the pigmented varieties strongly inhibited phorbol ester-induced tumor promotion in marmoset lymphoblastoid cells B95-8 in vitro. The information should be useful for applying the short-term test of anti-tumor-promoting activities of rice bran to other natural products and for evaluating pigmented rice cultivars as a genetic resource for the development of health-promoting rice varieties.

Last Modified: 9/29/2014
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