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United States Department of Agriculture

Agricultural Research Service

Title: Parallel Gene Expression Analysis in Cereal Development

item Druka, Arnis - SCOTTISH CROP RES. INST.
item Muehlbauer, Gary - UNIVERSITY OF MINNESOTA
item Wise, Roger
item Close, Timothy - UNIV. OF CALIFORNIA
item Kleinhofs, Andris - WASHINGTON STATE UNIV.
item Graner, Andreas - IPK, GERMANY
item Schulman, Alan - UNIV. OF HELSINKI
item Langridge, Peter - UNIV. OF ADELAIDE
item Sato, Kazuhiro - OKAYAMA UNIV., JAPAN
item Hayes, Patrick - OREGON STATE UNIV.
item Marshall, David - SCOTTISH CROP RES. INST.
item Waugh, Robbie - SCOTTISH CROP RES. INST.

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: January 10, 2004
Publication Date: January 10, 2004
Citation: Druka, A., Muehlbauer, G., Caldo, R., Wise, R.P., Close, T., Kleinhofs, A., Graner, A., Schulman, A., Langridge, P., Sato, K., Hayes, P., Marshall, D., Waugh, R. 2004. Parallel gene expression analysis in cereal development. Plant and Animal Genome XII Conference. p. 783.

Technical Abstract: Barley, Hordeum vulgare L., is particularly well suited for investigating patterns in monocot development. The recent release of the 22K Affymetrix Barley1 GeneChip probe array provides the opportunity to examine gene expression throughout the life cycle of a major cereal crop. In order to provide a reference data set for future investigations and hypothesis testing, transcriptional profiles of ca 22,700 barley genes were examined for 8 developmental stages and 15 tissue types in three independent replications. Additionally, identification of immediate genetic targets and tissue-specific studies for functions of unknown genes is also addressed. The implications of experimental design, technical procedures and the methodology of the data analysis will be discussed.

Last Modified: 4/18/2015
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