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ARS Home » Research » Publications at this Location » Publication #168880
Title: CLONING AND SEQUENCING OF THE BOVINE FAS-ASSOCIATED DEATH DOMAIN PROTEIN (FADD) CDNA

Author
item Connor, Erin
item Szperka, Michael
item Bannerman, Douglas

Submitted to: Germplasm Release
Publication Type: Germplasm Release
Publication Acceptance Date: 9/15/2004
Publication Date: 9/15/2004
Citation: Connor, E.E., Szperka, M.E., Bannerman, D.D. 2004. Cloning and sequencing of the bovine fas-associated death domain protein (fadd) cdna. Germplasm Release. Accession Number AY725483.

Interpretive Summary: This is a Germplasm Release. No interpretive Summary required.

Technical Abstract: Fas-associated death domain protein (FADD) is an adapter molecule expressed in many tissues that mediates cell apoptotic signaling. The full-length sequence of the bovine FADD cDNA was determined by reverse-transcription PCR using mRNA isolated from cultured bovine aortic endothelial cells and cloning of the resulting product into a plasmid vector for DNA sequencing. Sequencing of bovine FADD cDNA revealed approximately 83 and 80% nucleotide sequence identity to its human and murine homologues, respectively. A bovine FADD dominant-negative construct was developed to examine bacterial lipopolysaccharide (LPS)- and TNF-a-induced apoptosis in bovine endothelial cells. GenBank Accession No. AY725483.