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United States Department of Agriculture

Agricultural Research Service

Title: Apoptosis and Porcine Reproductive and Respiratory Syndrome Virus

Authors
item Miller, Laura
item Fox, James
item Chitko Mckown, Carol
item Laegreid, William

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: September 10, 2004
Publication Date: November 1, 2004
Citation: Miller, L.C., Fox, J.M., Chitko Mckown, C.G., Laegreid, W.W. 2004. Apoptosis and porcine reproductive and respiratory syndrome virus [abstract]. Research Workers in Animal Diseases Conference. p. 101.

Technical Abstract: Despite numerous studies examining the possible induction of apoptosis in Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infected cells, it remains unclear if PRRSV infection results in direct apoptotic induction. Studies examining the induction of the apoptotic gene expression response to PRRSV infection are needed to determine if PRRSV replication triggers an apoptotic response. We have utilized microarray and semi-quantitative reverse-transcription polymerase chain reaction (sqRT-PCR) to evaluate apoptotic gene expression in PRRSV-infected MARC-145 cells. Twenty-six apoptosis-related genes were examined during the first 24 hours of infection and found to be unaltered, indicating that apoptotic induction was not occurring in PRRSV infected cells. Additionally, using detection of free nucleosomal complexes, we examined cells for both apoptotic and necrotic death resulting from PRRSV infection at varying multiplicities of infection. This study indicates that PRRSV-infected MARC-145 cells undergo necrosis at a much higher level than apoptosis, and increases with virus levels used to infect the cells

Last Modified: 4/18/2014
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