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Title: CROSS REACTION OF ANTI-HUMAN CD MONOCLONAL ANTIBODIES ON SWINE CELLS: A SUMMARY OF THE SWINE SECTION OF THE HLDAS ANIMAL HOMOLOGUES DATA

Author
item Lunney, Joan
item DOMINGUEZ, J - MADRID, SPAIN
item ALVAREZ, B - MADRID, SPAIN
item REVELLA, C - MADRID, SPAIN
item EZQUERRA, A - MADRID, SPAIN
item ALONSO, F - MADRID, SPAIN
item HAVERSON, K - BRISTOL, UK
item GOLDE, WILLIAM - 1940-01-05
item Kuhar, Daniel
item FALDYNA, M - INDIANAPOLIS, IN

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 9/10/2004
Publication Date: 11/10/2004
Citation: Lunney, J.K., Dominguez, J., Alvarez, B., Revella, C., Ezquerra, A., Alonso, F., Haverson, K., Golde, W.T., Kuhar, D.J., Faldyna, M. 2004. Cross reaction of anti-human cd monoclonal antibodies on swine cells: a summary of the swine section of the hldas animal homologues data. Meeting Abstract.

Interpretive Summary:

Technical Abstract: The latest human lekocyte differentiation antigen (HLDA8) workshop included an Animal Homologues section. Participating laboratories anayzed the reactivity of 386 commercially available mAb against human CD molecules from various companies with cells from 17 different species, including swine cells, as reported here. All mAb were screened for + reactivity using flow cytometry on appropriate cell targets; ~80 of the mAb proved to be + on pig cells and are now being analyzed in more depth by confirming appropriate tissue and cell subset expression, effect of activation on expression, mw of antigen, and, if available, reaction with cloned swine gene product. To date, it is clear that certain anti-human CD1b, CD14, CD18, CD44, CD45, CD47, CD49c, CD61, CD68, CD91, CD95, CD163 and CD172a mAb exhibit clear cross reactions with swine cells. Some of these mAb will prove to be unique tools for swine immune studies; otehrs will also help address an aim of this HLDA8 section, i.e., to identify mAbs which recognize conserved, species independent CD epitopes. These results will contribute to the enhancement of the availability of mAbs and tools in veterinary medicine and immunology. Supported by the Veterinary Immunology Committee of IUIS.