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United States Department of Agriculture

Agricultural Research Service

Title: IGF-I AND IGF-II MRNA EXPRESSION IN SLOW AND FAST GROWING FAMILIES OF USDA103 CHANNEL CATFISH (ICTALURUS PUNCTATUS)

Authors
item PETERSON, BRIAN
item WALDBIESER, GEOFFREY
item BOURGEOIS, LANIE

Submitted to: Comparative Biochemistry and Physiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 23, 2004
Publication Date: November 1, 2004
Citation: Peterson, B.C., Waldbieser, G.C., Bilodeau, A.L. 2004. IGF-I and IGF-II mRNA expression in slow and fast growing families of USDA103 channel catfish (Ictalurus punctatus). Comparative Biochemistry and Physiology 139:317-323.

Interpretive Summary: Research was conducted to examine insulin like growth factor I (IGF-I) and insulin like growth factor II (IGF-II) messenger RNA (mRNA) expression in families of USDA103 channel catfish. Growth studies were conducted to determine the fastest and slowest growing families of fish. Family A was designated as the fastest growing while family H was designated as the slowest growing. Muscle and liver expression of IGF-I were similar between families. Expression of IGF-II mRNA was 3.3 and 1.8 fold greater in muscle and liver of the fast growing family compared to the slow growing family. In all families, expression of IGF-II mRNA was 12.2 fold greater in muscle and 5.8 fold greater in liver compared to expression of IGF-I mRNA. Elevated levels of IGF-II mRNA in muscle and liver compared to IGF-I mRNA, as well as differences in expression of IGF-II between fast and slow growing families of fish provide evidence for a role of IGF-II in growth of channel catfish.

Technical Abstract: Research was conducted to examine IGF-I and IGF-II mRNA expression in families of channel catfish. Relative expression of IGF-I and IGF-II mRNA was determined by real-time PCR. Family A exhibited a specific growth rate (SGR) of 3.6 and was designated as fast growing while family H exhibited a SGR of 3.1 and was designated as slow growing (P = 0.017). Expression of IGF-II mRNA was 3.3 (P = 0.006) and 1.8 (P = 0.049) fold greater in muscle and liver, respectively, for the fast growing family compared to the slow growing family. Expression levels of IGF-II mRNA were 12.2 fold greater (P < 0.001) in muscle and 5.8 fold greater (P = 0.021) in liver, respectively, compared to expression of IGF-I mRNA. Muscle and liver expression of IGF-I were similar between families. Elevated levels of IGF-II mRNA in muscle and liver compared to IGF-I mRNA, as well as differences in expression of IGF-II between fast and slow growing families of fish provide evidence for a role of IGF-II in growth of channel catfish.

Last Modified: 8/27/2014
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