Technical Abstract: Programmed cell death (PCD), as the name implies, is a genetic program that allows certain cells to die at a specific time and place in the body to allow normal development in all living organisms. Failed PCD can have serious consequences including disease and abnormal development. Despite its importance there is no comprehensive set of methods to study this phenomenon at cellular level in plants. A collaborative study between scientists from University of Ljubljana, Slovenia and Crop Genetics & Environmental Research Unit at Gainesville, led to the development of several such methods to study PCD in crop plants. The methods described here are well tested in the investigators' laboratory, can be used on microscopic quantity of specimen and will allow the study of PCD in a single cell to numerous cells inside a tissue (in situ). These methods will greatly facilitate detection of PCD in plants that may occur due to various stimuli, including the environmental stress.