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United States Department of Agriculture

Agricultural Research Service

Title: Fine-Mapping Milk Production Qtl on Bta6: Analysis of the Bovine Osteopontin Gene

Authors
item Schnabel, Robert - UNIVERSITY OF MISSOURI
item Ashwell, Melissa - NORTH CAROLINA STATE UNIV
item Sonstegard, Tad
item Connor, Erin
item Van Tassell, Curtis
item Kim, Jong-Joo - UNIVERSITY OF ARIZONA
item Taylor, Jeremy - UNIVERSITY OF MISSOURI

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: November 20, 2004
Publication Date: January 14, 2005
Citation: Schnabel, R.D., Ashwell, M.S., Sonstegard, T.S., Connor, E.E., Van Tassell, C.P., Kim, J., Taylor, J.F. 2005. Fine-mapping milk production qtl on bta6: analysis of the bovine osteopont [abstract]. Plant and Animal Genome XIII. p. 532.

Interpretive Summary: THIS IS AN ABSTRACT ONLY. NO INTERPRETIVE SUMMARY REQUIRED.

Technical Abstract: BTA6 harbors multiple QTL affecting milk production traits in dairy cattle. In order to fine-map these QTL, 3317 bulls comprising 45 half-sib families were genotyped for 38 markers. The data were analyzed using least squares regression (QTL Express), linkage disequilibrium (LDVCM) and full pedigree MCMC (LOKI) methods. A total of 19 sires were segregating for at least one QTL under a half-sib model at chromosome-wide P<0.05. LD results across families indicated the presence of up to 7 QTL (3 within a 6cM region) whereas LOKI revealed only 3 QTL. Positional/functional candidate genes have been identified for five of the QTL. Osteopontin (OPN or SPP1) is a strong candidate for the QTL near marker BM143. The entire OPN gene and 5kb upstream was sequenced from four segregating sires and four non-segregating sires (12.3kb per animal). A total of 15 SNPs were identified but only one SNP resulted in sire genotypes that were concordant with the segregation status of all eight sires.

Last Modified: 10/25/2014
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