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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Publications at this Location » Publication #171969
Title: MULTI-LOCUS SIMPLE SEQUENCE REPEAT (SSR) MARKERS FOR DIFFERENTIATING STRAINS AND EVALUATING GENETIC DIVERSITY OF XYLELLA FASTIDIOSA

Author
item Lin, Hong
item Civerolo, Edwin
item WALKER, ANDREW - UNIV OF CALIF-DAVIS
item HU, RONG - UNIV OF CALIF-DAVIS
item BARROS, SAMUEL - UNIV OF CALIF-DAVIS
item FRANCIS, MARTA - UNIV OF CALIF-DAVIS

Submitted to: Applied and Environmental Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/22/2005
Publication Date: 8/1/2005
Citation: Lin, H., Civerolo, E.L., Walker, A., Hu, R., Barros, S., Francis, M. 2005. Multi-locus simple sequence repeat (ssr) markers for differentiating strains and evaluating genetic diversity of xylella fastidiosa. Applied and Environmental Microbiology. 71:4888-4892.

Interpretive Summary: We designed and developed PCR-based DNA detection system called Simple Sequence Repeat (SSR) DNA markers to differentiate 43 Xylella fastidiosa (Xf) isolates collected from grape, citrus, almond and oleander plants. 34 designed SSR markers show powerful in discriminating genetically close Xf strains. This DNA-based detection system is a precise and repeatable. It useful tool for genetic diversity and population structure studies.

Technical Abstract: A genome wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases for four strains of Xylella fastidiosa (strains causing Pierce's disease, citrus variegated chlorosis, almond leaf scorch and oleander leaf scorch). Thirty-four SSR loci were selected for SSR primer design. These primers detected varying levels of polymorphism among 43 X. fastidiosa isolates derived from grape, citrus, almond and oleander. These multi-locus SSR primers are distributed across the X. fastidiosa genome and are useful for differentiating among and within host-associated X. fastidiosa isolates and for genetic diversity and population structure studies.