Submitted to: Genbank
Publication Type: Abstract Only
Publication Acceptance Date: December 7, 2004
Publication Date: December 12, 2004
Citation: Richards, M.P., Poch, S.M., Clarke, S.M., McMurtry, J.P. Meleagris gallopavo insulin-like growth factor-II (IGF-II) precursor gene, intron 3. GenBank 2004. Accession No. AY829237.
Primer-directed polymerase chain reaction (PCR) was used to completely sequence 713 bp of the third intron contained in the turkey insulin-like growth factor-II (IGF-II) gene from genomic DNA samples. The turkey IGF-II gene codes for a 187 amino acid pre-proprotein that includes a signal peptide, the mature IGF-II hormone, and a C-terminal extension peptide comprised of 24, 67 and 96 amino acids, respectively. Surprisingly, turkey IGF-II intron 3 showed a high degree (approximately 90%) of nucleotide sequence homology with the corresponding region of the chicken IGF-II gene. Typically, intronic sequence demonstrates a great deal of variation even among closely related species such as chickens and turkeys. This sequence data will be useful in studying the structure and expression of the turkey IGF-II gene in poultry.