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United States Department of Agriculture

Agricultural Research Service

Title: Molecular Typing Methods for Tracking Pathogens

Author
item Hiett, Kelli

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: May 10, 2005
Publication Date: July 15, 2005
Citation: Hiett, K.L. 2005. Molecular typing methods for tracking pathogens. In:J. Sofos (ed.), Raw Material Safety: Meat. Woodhead Publishing Limited. Cambridge, England. p. 592-605.

Interpretive Summary: The ability to determine the relatedness of pathogens in agricultural settings is becoming increasingly important for epidemiological investigations and for the subsequent development of intervention strategies to eliminate these pathogens from the food supply. There are several parameters to consider when choosing a typing method. One of the most critical considerations is the clonal relatedness of the organism for interest. Additional considerations include discriminatory power of the chosen technique, simplicity in performing the technique, reproductibility, throughout potential, and cost. Several phenotypic and genotypic techniques are discussed in further detail.

Technical Abstract: The ability to determine the relatedness of pathogens in agricultural settings is becoming increasingly important for epidemiological investigations and for the subsequent development of intervention strategies to eliminate these pathogens from the food supply. There are several parameters to consider when choosing a typing method. One of the most critical considerations is the clonal relatedness of the organism for interest. Additional considerations include discriminatory power of the chosen technique, simplicity in performing the technique, reproductibility, throughout potential, and cost. Several phenotypic and genotypic techniques are discussed in further detail.

Last Modified: 12/20/2014
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