Submitted to: Plant Biotechnology in Human Drug and Vaccine Development
Publication Type: Book / Chapter
Publication Acceptance Date: March 1, 2004
Publication Date: N/A
Technical Abstract: Plant viruses have been developed into transient expression vectors for the production of recombinant therapeutics in various plant systems. The earliest vectors were derived from viruses containing DNA genomes; however, plus-strand RNA viruses have been used with greater success despite early doubts of their sequence stability. Multiple expression strategies have been developed for the production of biologically active recombinant antibodies, vaccines, and other therapeutic proteins, for human and animal use. These include gene replacement, a duplicated subgenomic RNA promoter, gene fusions, and functional complementation. Transient expression from viral vectors allows easy scale up in agricultural settings and biological containment possibilities if a suitable vector is chosen. Regulatory issues and technical challenges will need to be addressed to fully realize the potential of plant virus-based vectors and the products produced in plants using this technology.