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Title: IDENTIFICATION OF BORDETELLA AVIUM BY PCR AND ANALYSIS OF AMPLICON SEQUENCE

Author
item Register, Karen
item Yersin, Andrew

Submitted to: Meeting Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 2/7/2005
Publication Date: 7/17/2005
Citation: Register, K.B., Yersin, A.G. 2005. Identification of Bordetella Avium by PCR and Analysis of Amplicon Sequence. 2005 AVMA/AAAP Annual Meeting, July 17-20, 2005, Minneapolis, Minnesota. 2005 CDROM.

Interpretive Summary:

Technical Abstract: Despite increased use and superior performance of PCR assays in clinical laboratories, identification of B. avium still depends upon isolation and biochemical testing. A B. avium-specific PCR has previously been reported, but details critical to reproducing it are not provided and analytical verification was not carried out. We have optimized reaction conditions using the previously described primer set and report 100% assay sensitivity and 98.6% specificity, based on analysis of 72 B. avium isolates and 85 other poultry isolates of various genera and species. The assay can be carried out on purified DNA or on boiled lysates prepared from single colonies. Sequence analysis of the amplicons generated from the B. avium isolates revealed occasional polymorphisms, one of which may be host-specific. The sequence data provides evidence for transmission of B. avium from wildlife to domesticated turkey flocks.