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United States Department of Agriculture

Agricultural Research Service

Title: Biolistic Transformation of Easter Lily Is Dependent on Callus Type

Authors
item Kamo, Kathryn
item Han, Bong Hee - RUR DEV ADMIN, KOREA

Submitted to: Society for In Vitro Biology Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: March 31, 2005
Publication Date: May 15, 2005
Citation: Kamo, K. 2005. Biolistic transformation of Easter lily is dependent on callus type. In Vitro Cell Deveel Biol-Plant. 41:55A (Abstract).

Technical Abstract: Two types of callus, a fast-growing friable callus and a slow-growing compact callus, were initiated from bulb scales of Lilium longiflorum cv. Nellie White. Sucrose, 3-12%, affected induction of friable callus but not compact callus. Friable callus was initiated when bulb scales were cultured on MS medium with 2 mg/L dicamba and 9% sucrose. Friable callus grew 5X faster than compact callus. There were many more plants regenerated from compact callus than friable callus, and these plants were phenotypically normal when grown to the flowering stage. Friable callus formed numerous somatic embryos when the callus was cultured on MS basal salts medium with 1% activated charcoal, but only a few somatic embryos germinated to form plants. Regeneration from compact callus appeared to be by organogenesis. Transformation was never achieved using friable callus that had been bombarded with either bar and uidA genes under the CaMV 35S promoter or nptII and uidA genes under the CaMV 35S promoter. One month following bombardment of friable callus, the number of gus positive cells declined significantly. Many transformed plants were selected from compact callus bombarded with these same plasmids indicating the benefit of using compact callus for transformation of Easter lily.

Last Modified: 9/10/2014
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