Technical Abstract: Garlic (Allium sativum) is the second most economically important Allium in the United States after onion and has been by necessity propagated by asexual means. Because of its obligate vegetative reproduction, no reports are available on genetic studies in garlic. In the 1980's, some male-fertile garlic accessions were discovered. These plants allowed for the beginning of genetic analysis and improvement of garlic. The goals of the research are to develop a linkage map of garlic using PCR-based DNA markers (SNP, indel, etc.) and to tag male fertility genes. The mapping population consists of 84 S1 progenies derived from one plant of PI 540316. Progenies segregate for male fertility, bulb size, numbers of leaves, stalk height, petal and anther color. Primers were designed from 133 unique onion ESTs and used to amplify DNA fragments from the parental plant. The DNA fragments were cloned and sequenced; amplicons were analyzed for the presence of SNPs, indels, and restriction enzyme site polymorphisms. In the poster, the molecular marker types detected and construction of the linkage map in garlic are discussed.