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United States Department of Agriculture

Agricultural Research Service

Title: Sub-Cellular Expression of a Species Cytoplasm Specific (Scs) Gene in Different Stages of Wheat Development.

Authors
item Hossain, Khwaja - NORTH DAKOTA STATE UNIV
item L Chingcuanco, Debbie
item Kalavacharla, Venugopal - NORTH DAKOTA STATE UNIV
item Riera-Lizarazu, Oscar - OREGON STATE UNIVERSITY
item Maan, Schivcharan - NORTH DAKOTA STATE UNIV
item Gonzalez-Hernandez, Jose - NORTH DAKOTA STATE UNIVER
item Kianian, Shahryar - NORTH DAKOTA STATE UNIV

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: December 1, 2004
Publication Date: January 15, 2005
Citation: Hossain, K.G., Chingcuanco, D.L., Kalavacharla, V., Riera-Lizarazu, O., Maan, S.S., Gonzalez-Hernandez, J.L., Kianian, S.F. 2005. Sub-cellular expression of a species cytoplasm specific (scs) gene in different stages of wheat development. Proceedings of the Plant & Animal Genome XIII Conference, January 15-19, 2005, San Diego, CA. p. 258.

Technical Abstract: Wild relatives of crop represent a usual reservoir of genes for the improvement of pest resistance, grain quality, and agronomic fitness of any cultivated species. Genetic incongruence is an obstacle to gene exchange between incompatible species and genes affecting nuclear-cytoplasmic (NC) interactions are directly or indirectly involved. In wheat, genes involved in NC interactions have been identified and because of their specificity they are termed as species cytoplasm specific (scs) genes. Triticum species differ with regard to compatibility with alien cytoplasms. For example, the durum wheat genome is normally not compatible with the cytoplasm of Triticum longissimum. This incompatibility however, is overcome by the presence of chromosome 1D of T. aestivum carrying a scs (scsae) gene. The alloplasmic line is male sterile and whenever pollinated with a euplasmic durum line produces plump (with scs) and shriveled inviable (without scs) seeds. NC interactions are reportedly involved in mitochondrial transcription processing, post-transcriptional function, biochemical detoxification, and alteration of mitochondrial genome organization. Mitochondrial gene specific oligo-arrays were developed and hybridized with mRNA from plump and shriveled embryos, mature and immature dry seeds. Fourteen genes were identified up-regulated in shriveled embryo, five of which up-regulated in shriveled immature seeds and only one up-regulated shriveled mature seed. Most of these genes were found involved in ATP synthesis, ribose, oxidative phosphorylation pathways and mitochondrial membrane permeability. These findings provide an insight into the sub-cellular expression of genes involved in NC interaction in wheat and related grass species.

Last Modified: 11/27/2014
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