Submitted to: Phytopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 26, 2005
Publication Date: October 1, 2005
Citation: Brown, J.K., Idris, A.M., Ostrow, K., Goldberg, N., French, R.C., Stenger, D.C. 2005. Genetic and phenotypic variation of the pepper golden mosaic virus complex. Phytopathology 95:1217-1224. Interpretive Summary: Three isolates of the whitefly-transmitted Pepper golden mosaic virus (PepGMV) were characterized for variability in genetic composition, ability to cause disease on a variety of crop and weed species, and transmission by the sweet potato whitefly. For each isolate, the two DNA components of the viral genome were cloned and the complete nucleotide sequence of each determined. Comparisons of DNA sequences indicated that the three isolates may be considered as strains of a single species. Inoculation of cloned viral DNA onto plants resulted in viral infection and demonstrated that the PepGMV isolates differed in the type of disease symptoms. Whitefly transmission assays indicated that only two of the three isolates could be transferred from plant to plant by whiteflies. When different pairs of the two DNA components were mixed, the resulting infections on plants indicated that the severity and type of symptoms produced were determined by the viral DNA B component. Collectively, these results indicate that isolates of PepGMV have the capacity to exchange genetic material and that reassortment may lead to new variants that differ in the severity of disease produced on crop species.