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United States Department of Agriculture

Agricultural Research Service

Research Project: CHARACTERIZATION AND IMPROVEMENT OF SUGAR INDUSTRY PROCESS UNITS IMPACTED BY NEW PRODUCTION PRACTICES

Location: Commodity Utilization Research

Title: Uniform and Simple Measurement of the Activity of Dextranases at the Sugarbeet Factory

Author
item Eggleston, Gillian

Submitted to: American Society of Sugar Cane Technologists
Publication Type: Abstract Only
Publication Acceptance Date: April 28, 2005
Publication Date: June 22, 2005
Citation: Eggleston, G. 2005. Uniform and simple measurement of the activity of dextranases at the sugarbeet factory (abstract). American Society of Sugar Cane Technologists.

Technical Abstract: Currently, the activities or strengths of commercial dextranases in the U.S. cannot be directly compared because there is no uniform method used by vendors and/or distributors to measure the activity. A very wide variation exists in the activities of commercial dextranases, and this is compounded by the fact that activities and prices change regularly. Moreover, the factory storage characteristics of commercial dextranases differ enormously, which further highlights the need for not only a uniform method to (a) measure and economically compare the activities of different commercial dextranases, but (b) one which can be used simply at the sugarbeet factory to measure the activity of factory delivered batches of enzymes, and their changing activities on factory storage. A dextranase activity method, based on simple titration, was identified and modified for easy use a the sugarbeet factory. This method does not need any sophisticated equipment and there is no need for standards and a standard curve.

Last Modified: 4/24/2014
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