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United States Department of Agriculture

Agricultural Research Service

Title: Addition of Sulsamethoxazole to Selective Media Aids in the Recovery of Campylobacter Jejuni

Authors
item Line, John
item Bailey, Joseph

Submitted to: Campylobacter Helicobacter and Related Organisms International Workshop
Publication Type: Abstract Only
Publication Acceptance Date: June 15, 2005
Publication Date: September 6, 2005
Citation: Line, J.E., Bailey, J.S. 2005. Addition of sulsamethoxazole to selective media aids in the recovery of campylobacter jejuni. Campylobacter Helicobacter and Related Organisms International Workshop. 118

Technical Abstract: The purpose of our experiment was to determine the influence of sulsamethozazole (SMX) on recovery of C. jejuni from broiler carcass rinse samples when added to Campy-Cefex or Campy-Line agars (CLA). Cefex and CLA were prepared with and without the addition of 25 mg/L SMX. Broiler carcasses post chill were obtained from a local commercial processor and rinsed in 400 ml buffered peptone. Serial dilutions were surface plated on the selective agars and incubated for 48h at 42 degrees C under microaerobic conditions. Following incubation plates were inspected for growth and typical Campylobacter colonies and non-Campylobacter contaminants were differentially enumerated. Both Cefex and CLA with and without SMX recovered similar populations of Campylobacter; however, significantly fewer contaminants were observed on the Cefex with added SMX as compared to normal Cefex. Neither the normal CLA or CLA with SMX had contaminants present from this sample type due to the increased selectivity of the CLA as compared to Cefex. Addition of SMX should be considered for increasing selectivity of Campylobacter specific media.

Last Modified: 11/22/2014
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