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United States Department of Agriculture

Agricultural Research Service

Title: Complete Nucleotide Sequence of a Wisconsin Soyean Isolate of Soybean Dwarf Virus

Authors
item Domier, Leslie
item Veetil, Thanuja - UNIV OF ILLINOIS
item Phibbs, Annette - UNIV OF WISCONSIN
item Barta, Adrian - UNIV OF WISCONSIN

Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: August 4, 2005
Publication Date: August 4, 2005
Citation: Domier, L.L., Veetil, T.T., Phibbs, A., Barta, A. 2005. Complete nucleotide sequence of a Wisconsin soybean isolate of Soybean Dwarf Virus [abstract].American Phytopathological Society. 95:S25.

Technical Abstract: In 2003, Soybean dwarf virus (SbDV), which causes significant reductions in soybean (Glycine max) yields in Japan, was detected in soybean plants in four Wisconsin counties in association with high densities of soybean aphids (Aphis glycines). Even though SbDV was detected in 43% of red clover plants (Trifolium pratense) in Illinois, we failed to detect transmission of clover isolates of SbDV to or from soybean by A. glycines. To investigate possible changes in the transmission phenotypes of soybean isolates of SbDV, the complete nucleotide sequence was determined and cDNA clones were constructed from one Wisconsin soybean SbDV isolate. The 5709-nt sequence of the Wisconsin isolate was 96% identical to a dwarfing SbDV isolate from Japan that is transmitted most efficiently by Acyrthosiphon pisum. Comparisons of the predicted amino acid sequences of the Wisconsin and Japanese SbDV isolates revealed a single amino acid substitution in the 22-kDa coat protein and 36 substitutions in the 54-kDa readthrough domain. In other members of the Luteoviridae, the readthrough domain has been shown to be an important determinant in the specificity of aphid transmission. Hence, the higher density of amino acid substitutions in the readthrough domain may reflect adaptations to different vector populations.

Last Modified: 8/30/2014
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